Ava is a sub girl in bondage, allowing the doctor to use her body for his own pleasure in his awful office.

To save humanity, Dr. Horrible sets out to find out the truth after mistaking beautiful Ava for an alien or perhaps a human of "French descent." Despite his successful completion of medical school, he was able to preserve an unusual collection of irrelevant information. What is Ava going to do? Will she be able to persuade him that she is just as human as he is, albeit much more sensible?